Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[DNA typing methods of HLA].

K Kashiwase1

  • 1Japanese Red Cross Central Blood Center.

Rinsho Byori. the Japanese Journal of Clinical Pathology
|September 4, 1999
PubMed
Summary

Human leukocyte antigens (HLA) are polymorphic cell membrane proteins crucial for immune response. DNA-based typing methods have been developed for accurate HLA allele identification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HLA-DPB1 mismatch induces a graft-versus-leukemia effect without severe acute GVHD after single-unit umbilical cord blood transplantation.

Leukemia·2017
Same author

A genome-wide association study identifies two novel susceptibility loci and trans population polygenicity associated with bipolar disorder.

Molecular psychiatry·2017
Same author

A novel HLA-DQB1*04 allele, DQB1*04:10, identified in a Japanese individual.

Tissue antigens·2013
Same author

Identification of a novel HLA allele, HLA-DQB1*06:51, in a Japanese rheumatoid arthritis patient.

Tissue antigens·2012
Same author

Associations between six classical HLA loci and rheumatoid arthritis: a comprehensive analysis.

Tissue antigens·2012
Same author

Application of bead array technology to simultaneous detection of human leucocyte antigen and human platelet antigen antibodies.

Vox sanguinis·2009

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Human Genetics

Context:

  • Human leukocyte antigens (HLA) are highly polymorphic proteins essential for immune regulation.
  • The HLA gene complex on chromosome 6 encodes these critical cell membrane proteins.
  • Over 650 alleles have been identified across HLA-A, B, C, and DR loci.

Purpose:

  • To review the significance of HLA polymorphism in immune response.
  • To discuss the development of DNA-based typing methods for HLA alleles.
  • To highlight the availability of automated and user-friendly HLA typing techniques.

Summary:

  • HLA proteins mediate self/not-self discrimination, a fundamental aspect of the immune system.
  • Various DNA-based typing methodologies, including those using specific primers, probes, or restriction enzymes, have been established.
  • Current advancements offer simple, automated methods for precise HLA allele determination.

Impact:

  • Facilitates deeper understanding of immune system function and diversity.
  • Enables advancements in transplantation and disease association studies.
  • Improves the efficiency and accessibility of HLA typing for research and clinical applications.

Related Experiment Videos