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Related Experiment Videos

Calcium-induced ERK activation in human T lymphocytes.

P A Atherfold1, M S Norris, P J Robinson

  • 1Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27858, USA.

Molecular Immunology
|September 4, 1999
PubMed
Summary

Calcium ionophores activate ERK1 and 2 in T lymphocytes, revealing a new role for calcium signaling in these cells. This rapid, dose-dependent activation is blocked by MEK inhibitors, highlighting a novel pathway.

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Area of Science:

  • Immunology
  • Cell Signaling
  • Molecular Biology

Background:

  • Previous studies demonstrated calcium ionophore-induced ERK2 activation in B lymphocytes.
  • The role of calcium signaling in T lymphocyte activation remained less understood.

Purpose of the Study:

  • To investigate the effect of calcium ionophores on ERK1 and ERK2 activation in human primary and Jurkat T lymphocytes.
  • To elucidate the signaling pathways involved in calcium-mediated T lymphocyte activation.

Main Methods:

  • Stimulation of T lymphocytes with calcium ionophores (ionomycin and A23187).
  • Assessing ERK1/2 activation via SDS-PAGE mobility shifts, phospho-specific antibody binding, and myelin basic protein (MBP) phosphorylation assays.
  • Inhibition studies using the MEK inhibitor PD98059.

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Main Results:

  • Ionomycin and A23187 rapidly and transiently activated ERK1 and ERK2 in T lymphocytes in a dose-dependent manner.
  • Activation was confirmed by multiple biochemical assays, including increased MBP phosphorylation.
  • MEK inhibitor PD98059 blocked calcium ionophore-induced ERK1/2 activation.

Conclusions:

  • Calcium fluxes play a significant role in T lymphocyte activation through the ERK1/2 pathway.
  • This study identifies a novel signaling mechanism involving calcium and ERK activation in T cells.