Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Delayed nucleoid segregation in Escherichia coli.

P G Huls1, N O Vischer, C L Woldringh

  • 1Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands.

Molecular Microbiology
|September 4, 1999
PubMed
Summary

Cell division protein PbpB is crucial for nucleoid segregation in Escherichia coli. Defects in PbpB delay DNA segregation, impacting cell division and overall bacterial growth.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probing plasma membrane microdomains in cowpea protoplasts using lipidated GFP-fusion proteins and multimode FRET microscopy.

Journal of microscopy·2004
Same author

Polymer-mediated compaction and internal dynamics of isolated Escherichia coli nucleoids.

Journal of structural biology·2002
Same author

Isolation of the Escherichia coli nucleoid.

Biochimie·2001
Same author

HU-GFP and DAPI co-localize on the Escherichia coli nucleoid.

Biochimie·2001
Same author

Experiments on movement of DNA regions in Escherichia coli evaluated by computer simulation.

Biochimie·2001
Same author

Perpendicular planes of FtsZ arcs in spheroidal Escherichia coli cells.

Biochimie·2001

Area of Science:

  • Microbiology
  • Molecular Biology
  • Cell Biology

Background:

  • Nucleoid segregation is essential for bacterial cell division.
  • The precise mechanisms governing nucleoid segregation remain incompletely understood.
  • Cell division proteins are implicated in various stages of the cell cycle.

Purpose of the Study:

  • To investigate the role of cell division proteins in nucleoid segregation.
  • To determine the impact of specific mutations on DNA content and segregation timing.
  • To elucidate the function of PbpB in the context of bacterial DNA replication and division.

Main Methods:

  • Image cytometry was used to measure DNA content in Escherichia coli cell division mutants.
  • Analysis of nucleoid DNA content in isogenic strains, including pbpB(Ts) and ftsZ mutants.

Related Experiment Videos

  • Determination of C, D, and D' periods (replication, division, and segregation timing) using rifampicin inhibition.

    • Main Results:

    • Mutant strains (pbpB(Ts), ftsZ) exhibited increased DNA content per nucleoid compared to wild-type.
    • The D' period, representing segregation time, was significantly prolonged in pbpB(Ts) mutants at permissive temperatures.
    • These findings were independent of chromosome dimer formation.

    Conclusions:

    • Defective cell division protein PbpB directly impacts nucleoid segregation timing.
    • PbpB plays a role beyond cell division, influencing the final stages of DNA segregation.
    • PbpB is proposed to be involved in divisome assembly, which facilitates nucleoid segregation.