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High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells
R R Duncan1, A C Don-Wauchope, S Tapechum
1Membrane Biology Group, Biomedical Sciences, University of Edinburgh Medical School, Teviot Place, Edinburgh, EH8 9XD, U.K.
The Biochemical Journal
|September 8, 1999
Abstract:
Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.