Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Growth and differentiation regulate CD44 expression on human keratinocytes.

J Zhou1, J G Haggerty, L M Milstone

  • 1Department of Dermatology, Yale University School of Medicine and Veterans Affairs Medical Center, New Haven, Connecticut 06510, USA.

In Vitro Cellular & Developmental Biology. Animal
|September 9, 1999
PubMed
Summary

Cell growth and differentiation significantly alter CD44 receptor expression in keratinocytes. Confluence and differentiation downregulate many CD44 protein variants, with epican being predominant.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression and localization of thymidine phosphorylase/platelet-derived endothelial cell growth factor in skin and cutaneous tumors.

Journal of cutaneous pathology·1999
Same author

Beta-L-1,3-dioxolane-cytidine: a novel nucleoside that inhibits proliferation and induces differentiation of keratinocytes in vitro.

Skin pharmacology and applied skin physiology·1999
Same author

[The study of epidermal growth factor in acceleration of skin wound healing].

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery·1998
Same author

High mutation detection rate in the COL4A5 collagen gene in suspected Alport syndrome using PCR and direct DNA sequencing.

Journal of the American Society of Nephrology : JASN·1998
Same author

Evaluation of GM1 ganglioside-mediated apoptosis in feline thymocytes.

Veterinary immunology and immunopathology·1998
Same author

Effect of repeated stress on body weight and body composition of rats fed low- and high-fat diets.

The American journal of physiology·1998

Area of Science:

  • Dermatology
  • Cell Biology
  • Molecular Biology

Background:

  • CD44 receptors are crucial for cell adhesion and signaling in skin.
  • Understanding CD44 regulation in keratinocytes is key to skin biology.

Purpose of the Study:

  • To investigate factors regulating CD44 expression in keratinocytes.
  • To analyze CD44 protein variants under different growth and differentiation conditions.

Main Methods:

  • Western immunoblots and metabolic labeling were used.
  • Keratinocytes were studied in vitro and in isolated epidermis.
  • Analysis focused on preconfluent, confluent, and differentiated cells.

Main Results:

  • Keratinocyte CD44 expression patterns vary with growth and differentiation.

Related Experiment Videos

  • Proliferating cells show multiple CD44 variants; differentiated cells show fewer.
  • Epican is the dominant CD44 form, with size variations observed.

    • Conclusions:

    • Cell confluence and differentiation are major regulators of CD44 expression in keratinocytes.
    • Different CD44 protein forms suggest distinct functional roles.
    • Regulation of CD44 impacts keratinocyte behavior and skin physiology.