Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 11, 2026

Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers

Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers

Published on: May 28, 2007

Determining chemotactic responses by two subsurface microaerophiles using a simplified capillary assay method.

R Mazumder¹, T J Phelps, N R Krieg

¹Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA.

Journal of Microbiological Methods

|September 10, 1999

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nodding syndrome: A key role for sources of nutrition?

eNeurologicalSci·2022

Same author

Scanning window analysis of non-coding regions within normal-tumor whole-genome sequence samples.

Briefings in bioinformatics·2020

Same author

COVID-19 Biomarkers in research: Extension of the OncoMX cancer biomarker data model to capture biomarker data from other diseases.

bioRxiv : the preprint server for biology·2020

Same author

The etiology of nodding syndrome phenotypes remains unknown<sup>§,§§</sup>.

Revue neurologique·2020

Same author

Nodding syndrome phenotypes.

Revue neurologique·2019

Same author

Impact of germline and somatic missense variations on drug binding sites.

The pharmacogenomics journal·2016

A new, simplified capillary chemotaxis assay was developed for measuring bacterial tactic responses. This method proved more convenient than the Adler method and successfully characterized responses in two subsurface bacterial strains.

Area of Science:

Microbiology
Bacterial Physiology
Chemotaxis Assays

Background:

Bacterial chemotaxis is crucial for survival and colonization.
Existing methods for measuring chemotaxis can be complex and time-consuming.
Subsurface bacteria exhibit unique behaviors influenced by environmental gradients.

Purpose of the Study:

To develop a simplified and convenient capillary chemotaxis assay.
To measure and compare the chemotactic responses of two distinct subsurface bacterial isolates.
To validate the new assay against a standard technique.

Main Methods:

A simplified capillary chemotaxis assay was designed using a hypodermic needle, syringe, and pipette tip.
The assay was applied to two subsurface microaerophilic bacterial strains: Pseudomonas syringae (VT10) and a strain related to Pseudomonas mendocina (MR100).

More Related Videos

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems

Published on: May 5, 2020

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates

Published on: March 25, 2022

Related Experiment Videos

Last Updated: Jul 11, 2026

Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers

Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers

Published on: May 28, 2007

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems

Published on: May 5, 2020

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates

Published on: March 25, 2022

Chemotaxis results were verified using the swarm plate assay technique.

Main Results:

Isolate VT10 (Pseudomonas syringae) showed chemotactic attraction to dextrose, glycerol, phenol, and maltose.
Isolate MR100 (related to Pseudomonas mendocina) exhibited no tactic response to the tested compounds, despite utilizing some as carbon sources.
The simplified assay provided results consistent with the swarm plate assay.

Conclusions:

The developed simplified capillary chemotaxis assay is a convenient alternative to existing methods.
The study characterized differential chemotactic responses in two subsurface bacterial isolates.
This simplified technique holds potential for routine chemotactic testing in microbiology.