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Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

Clonal interference and the evolution of RNA viruses.

R Miralles1, P J Gerrish, A Moya

  • 1Institut Cavanilles de Biodiversitat i Biología Evolutiva and Departament de Genètica, Universitat de València, Apartado 22085, 46071 València, Spain.

Science (New York, N.Y.)
|September 11, 1999
PubMed
Summary

Clonal interference in asexual populations means beneficial mutations compete, leading to large-effect mutations and slower adaptation. This study quantified these effects in the vesicular stomatitis virus.

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Area of Science:

  • Evolutionary biology
  • Genetics
  • Virology

Background:

  • Asexual populations face competition from beneficial mutations in different lineages.
  • This competition, termed clonal interference, impacts mutation fixation and adaptation rates.
  • Understanding clonal interference is crucial for predicting evolutionary trajectories.

Purpose of the Study:

  • To measure the effects of clonal interference in a real biological system.
  • To quantify the rates and average effects of beneficial mutations under clonal interference.
  • To assess the impact of clonal interference on the speed of adaptation in asexual populations.

Main Methods:

  • Studied the asexual RNA virus, vesicular stomatitis virus.
  • Quantified the rates of beneficial mutations.
  • Measured the average effects of beneficial mutations that achieved fixation.

Main Results:

  • Clonal interference was observed to favor beneficial mutations of large effect.
  • The time between beneficial mutation fixations was increased due to clonal interference.
  • Adaptation rates in the asexual vesicular stomatitis virus population were slowed.

Conclusions:

  • Clonal interference significantly shapes the evolutionary dynamics of asexual populations.
  • The phenomenon leads to selection for large-effect beneficial mutations and decelerated adaptation.
  • Vesicular stomatitis virus serves as a model to demonstrate the quantitative impact of clonal interference.