Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epidermal growth factor pathway substrate 15, Eps15.

A E Salcini1, H Chen, G Iannolo

  • 1Department of Experimental Oncology, European Institute of Oncology, Milano, Italy.

The International Journal of Biochemistry & Cell Biology
|September 11, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of non-coding RNAs in age-related vascular cognitive impairment: An overview on diagnostic/prognostic value in Vascular Dementia and Vascular Parkinsonism.

Mechanisms of ageing and development·2020
Same author

The PU.1 transcription factor induces cyclin D2 expression in U937 cells.

Leukemia·2006
Same author

The Eps15 C. elegans homologue EHS-1 is implicated in synaptic vesicle recycling.

Nature cell biology·2001
Same author

Numb is an endocytic protein.

The Journal of cell biology·2000
Same author

Tyrosine phosphorylation of Eps15 is required for ligand-regulated, but not constitutive, endocytosis.

The Journal of cell biology·2000
Same author

The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport.

The Journal of cell biology·1999
Same journal

Corrigendum to "Sodium butyrate down-regulation of indoleamine 2, 3-dioxygenase at the transcriptional and post-transcriptional levels" [Int. J. Biochem. Cell Biol. 42 (2010) 1840-1846].

The international journal of biochemistry & cell biology·2026
Same journal

Whole-brain spatial metabolomics reveals metabolic gradient shifts in a murine glioma model following boron neutron capture therapy (130 characters).

The international journal of biochemistry & cell biology·2026
Same journal

LCN2 modulates Th17/Treg balance in vitro and is associated with an adaptive response to intestinal ischemia-reperfusion injury under Hmox1-deficient conditions.

The international journal of biochemistry & cell biology·2026
Same journal

Chloroquine modulates the redox-sensitive signalling via inhibiting the AMPK-ULK1 under LPS induced state in murine splenic macrophages.

The international journal of biochemistry & cell biology·2026
Same journal

The vicious cycle of hyperglycemia and oxidative stress: Novel mechanistic insights into a pathogenic alliance.

The international journal of biochemistry & cell biology·2026
Same journal

Fibroblast growth factors (FGF) in the pathogenesis and treatment of inflammatory bowel diseases (IBD): An overview.

The international journal of biochemistry & cell biology·2026
See all related articles

Eps15 protein is crucial for endocytosis, the process cells use to internalize molecules like epidermal growth factor (EGF). Its dysfunction is linked to cell proliferation control and cancer.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Eps15 protein identified as a substrate for epidermal growth factor receptor (EGFR) kinase activity.
  • Eps15 possesses a tripartite structure with EH domains, a coiled-coil region, and a DPF-repeat domain.
  • Eps15 localizes to clathrin-coated pits, interacting with AP-2 and Epsin.

Purpose of the Study:

  • To elucidate the role of Eps15 and Epsin in receptor-mediated endocytosis.
  • To explore the implications of EH-domain containing proteins in intracellular sorting.
  • To investigate the potential of Eps15 in cell proliferation and neoplasia.

Main Methods:

  • Investigated Eps15 and Epsin function in endocytosis.
  • Studied interactions between Eps15, AP-2, and Epsin.

Related Experiment Videos

  • Examined the role of Eps15 in cell transformation and its association with HRX/ALL/MLL gene rearrangements.

    • Main Results:

    • Perturbation of Eps15 and Epsin inhibits EGF and transferrin endocytosis, confirming their role in the endocytic machinery.
    • Eps15 demonstrates transforming potential in NIH-3T3 cells.
    • Eps15 gene rearrangements are observed in acute myelogenous leukemias.

    Conclusions:

    • Eps15 and Epsin are essential components of the cellular endocytic machinery.
    • Interfering with EH-domain protein function offers potential strategies for controlling cell proliferation.
    • Eps15 is implicated in the subversion of cell proliferation in cancer.