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Related Experiment Videos

Structural elements common to mitosis and apoptosis.

A Sesso1, D T Fujiwara, M Jaeger

  • 1Laboratory of Molecular Pathology, Faculty of Medicine of São Paulo, Brazil. antses88@uol.com.br

Tissue & Cell
|September 11, 1999
PubMed
Summary
This summary is machine-generated.

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Researchers identified a third similarity between cell mitosis and apoptosis: the presence of approximately 50 nm vesicles. These vesicular clusters, found in various rat and human cells undergoing these processes, are associated with the Golgi apparatus and rough endoplasmic reticulum.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Apoptosis Research

Background:

  • Mitotic and apoptotic cells share chromatin hypercondensation and nuclear envelope loss.
  • Existing research highlights similarities between mitosis and apoptosis.

Purpose of the Study:

  • To identify and describe a third common feature between mitotic and apoptotic cells.
  • To investigate the ultrastructural characteristics of vesicular elements during cell division and death.

Main Methods:

  • Electron microscopy was used to examine PC-12 lineage cells and various rat tissue cells undergoing apoptosis.
  • HeLa, WEHI-3, and HL-60 cells were treated with specific agents (TNF, VM 26, thapsigargin) to induce apoptosis.
  • Fluorescence microscopy with Hoechst stain and antibodies against Golgi proteins was employed to analyze chromatin and organelle distribution.

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Main Results:

  • Clusters of approximately 50 nm vesicles, alongside 100-200 nm tubules, were observed in apoptotic PC-12 cells and subsequently in various rat and human cell lines.
  • These vesicular clusters were also found in mitotic PC-12 cells, indicating a shared characteristic.
  • Fluorescence microscopy revealed a pattern compatible with vesicular clusters in apoptotic cells, often associated with the Golgi apparatus and rough endoplasmic reticulum.

Conclusions:

  • The presence of approximately 50 nm vesicular clusters is a third shared feature of mitosis and apoptosis.
  • These vesicles are associated with the Golgi apparatus and rough endoplasmic reticulum, suggesting a role in organelle dynamics during both processes.
  • The findings contribute to understanding the ultrastructural similarities between cell division and programmed cell death.