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Updated: Apr 13, 2026

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
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Store-operated Ca2+ entry: evidence for a secretion-like coupling model.

R L Patterson1, D B van Rossum, D L Gill

  • 1Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA.

Cell
|September 11, 1999
PubMed
Summary

The study reveals that the endoplasmic reticulum (ER) and plasma membrane (PM) communicate via a physical, reversible mechanism. Actin cytoskeleton rearrangements disrupt this calcium (Ca2+) signaling pathway, but its normal function can be restored.

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Area of Science:

  • Cell Biology
  • Calcium Signaling
  • Membrane Dynamics

Background:

  • The precise mechanisms linking endoplasmic reticulum (ER) calcium (Ca2+) stores to plasma membrane (PM) Ca2+ channels remain incompletely understood.
  • Store-operated calcium entry (SOCE) is a critical process regulated by the communication between ER Ca2+ stores and PM channels.

Purpose of the Study:

  • To investigate the role of the cytoskeleton, specifically actin filaments, in mediating the coupling between ER Ca2+ stores and PM Ca2+ channels.
  • To elucidate the physical basis of the communication between ER and PM for calcium signaling.

Main Methods:

  • Utilized cytoskeletal modifications, including actin filament disruption and redistribution, to probe ER-PM coupling.
  • Observed the effects of these modifications on Ca2+ entry channels and inositol 1,4,5-trisphosphate-mediated store release.

Main Results:

  • Disassembly of the actin cytoskeleton did not affect ER-PM coupling.
  • Redistribution of F-actin to the cell cortex displaced ER, disrupting coupling and preventing Ca2+ entry, without affecting inositol 1,4,5-trisphosphate-induced Ca2+ release.
  • Reversal of actin cortical layer formation restored ER-PM proximity and coupling.

Conclusions:

  • Calcium signaling coupling between ER and PM is mediated by a physical, secretion-like mechanism involving close, reversible interactions.
  • Actin cytoskeleton plays a crucial role in regulating the proximity and functional coupling of ER and PM for calcium entry.