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Updated: Aug 7, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
Published on: June 12, 2016
Empirical relationship between chiral selectivity and mobile phase modifier properties
J A Blackwell1, R W Stringham, D Xiang
1Dupont Pharmaceuticals Company, Chemical Process Research and Development, Chambers Works, Deepwater, NJ 08023, USA. john.a.blackwell@dupontpharma.com
Abstract:
An empirical relationship was derived which relates properties of the mobile phase modifier to the chiral selectivity factor for a given analyte/chiral selector combination. Using carbon dioxide and heptane-based mobile phases, the effect of various mobile phase modifiers on Pirkle-type stationary phases may be accurately modeled using a two-parameter equation. Similar results are obtained using cellulosic stationary phases with carbon dioxide-based mobile phases. Modeling separations performed using heptane-based mobile phases with cellulosic stationary phases were not successful. The predictive ability of this modeling approach was demonstrated using novel modifiers and chiral analytes.
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