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Related Experiment Videos

Affinity purified human antiphospholipid antibodies bind normal term placenta.

S Donohoe1, J C Kingdom, I J Mackie

  • 1Department of Hematology, Watford General Hospital, University College London, UK.

Lupus
|September 14, 1999
PubMed
Summary

Antiphospholipid antibodies (aPL) can bind directly to placental tissues, potentially causing damage and fetal loss. This study demonstrates aPL binding to human placenta, suggesting a mechanism for pregnancy complications.

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Area of Science:

  • Obstetrics and Gynecology
  • Immunology
  • Pathophysiology

Background:

  • Antiphospholipid antibodies (aPL) are linked to increased fetal loss.
  • The exact mechanism by which aPL cause placental damage is not fully understood.
  • Direct binding of aPL to the placenta may lead to thrombosis and infarction.

Purpose of the Study:

  • To investigate the direct binding of human aPL to human placental tissue.
  • To explore the potential role of aPL in mediating placental damage.

Main Methods:

  • Development of an immunofluorescent technique to detect aPL binding to placenta.
  • Affinity purification of aPL from patient sera using cardiolipin and phosphatidylserine liposomes.
  • Preliminary SDS-PAGE and western blotting to identify bound placental proteins.

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Main Results:

  • Human aPL demonstrated heterogeneous binding to normal term placenta in five of six patients.
  • Binding occurred at the trophoblast microvillous surface, stromal, and peri-vascular regions.
  • Preliminary studies indicated aPL bind to various placental proteins, with beta2GPI not being predominant.

Conclusions:

  • This study provides evidence that aPL can bind directly to human placental tissue.
  • Direct placental binding of aPL may be a key mechanism contributing to fetal loss in antiphospholipid syndrome.