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Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
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Published on: September 9, 2011

Antigen-driven clonal accumulation of peritoneal gammadelta T cells in vivo

Y Kodaira1, K Ikuta, S Tanaka

  • 1Department of Microbiology & Immunology, Nippon Medical School, Tokyo, Japan. kodaira.yuzo@mayo.edu

Immunological Investigations
|September 15, 1999
PubMed

How the clonality of gammadelta T cells changes in response to exogenous antigens is uncertain. Here we analyzed kinetics of Vgamma1.1 and Vgamma2 T cell clonality after intraperitoneal injection of purified protein derivatives (PPD) by the heterogeneity of the third complementarity determining region (CDR3) length in Vgamma1.1-Jgamma4-Cgamma4 and Vgamma2-Jgamma1-Cgamma1 junctions. The V-J junctions were analyzed in intrahepatic lymphocytes (IHL), spleen cells, and peritoneal exudate cells (PEC) by polyacrylamide gel electrophoresis. Gammadelta T cells expressing Vgamma1.1 and Vgamma2 genes were heterogeneous in normal mice. Accumulation of specific Vgamma1.1 T cell clones was transiently detected 7 days after the injection in PEC, but no accumulation was observed in IHL and spleen cells. The accumulated clones disappeared by 4 weeks. Transient accumulation of Vgamma2 T cell clones was also observed in PEC at the early phase after the injection. These results suggest that gammadelta T cells with specific TCR respond to PPD and temporary accumulate in the peritoneal cavity, but not in liver and spleen.

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