Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Homing endonucleases: structure, function and evolution.

M S Jurica1, B L Stoddard

  • 1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98109, USA.

Cellular and Molecular Life Sciences : CMLS
|September 16, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hit-and-run programming of therapeutic cytoreagents using mRNA nanocarriers.

Nature communications·2017
Same author

Crystallization and preliminary X-ray analysis of bacterial cytosine deaminase.

Acta crystallographica. Section D, Biological crystallography·2001
Same author

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.

Nucleic acids research·2001
Same author

Structure of a factor VIII C2 domain-immunoglobulin G4kappa Fab complex: identification of an inhibitory antibody epitope on the surface of factor VIII.

Blood·2001
Same author

Trapping reaction intermediates in macromolecular crystals for structural analyses.

Methods (San Diego, Calif.)·2001
Same author

The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sites.

Nature structural biology·2001
Same journal

Single-Cell dissection of fibrodysplasia ossificans progressiva identifies SPP1 as a mediator of macrophage-fibroadipogenic progenitors crosstalk.

Cellular and molecular life sciences : CMLS·2026
Same journal

Ablation of the renal tubular gluconeogenic enzyme PCK1 drives AKI-to-CKD transition by negatively regulating the TGF-β/Smad3 signaling pathway.

Cellular and molecular life sciences : CMLS·2026
Same journal

LncRNA modulates Dpp-mediated wing development to influence flight in Aedes aegypti.

Cellular and molecular life sciences : CMLS·2026
Same journal

TROP2 promotes bone metastasis of colorectal cancer through interaction with the fibronectin-integrin axis.

Cellular and molecular life sciences : CMLS·2026
Same journal

PRMT5-Cacna1d axis maintains calcium homeostasis to regulate postnatal motor development in mice.

Cellular and molecular life sciences : CMLS·2026
Same journal

Advances and clinical potential of epigenome editing.

Cellular and molecular life sciences : CMLS·2026
See all related articles

Homing is a genetic process where intervening sequences duplicate. Homing endonucleases, enzymes initiating this process, are key to understanding DNA repair and gene transfer.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Homing is the lateral transfer of genetic sequences like introns or inteins.
  • This process results in the duplication of the intervening sequence.
  • Site-specific endonucleases, encoded within mobile elements, initiate homing.

Purpose of the Study:

  • To explore the structural and functional characteristics of homing endonucleases.
  • To compare homing endonucleases with other DNA-modifying enzymes.
  • To investigate the unique DNA recognition strategies employed by these enzymes.

Main Methods:

  • Analysis of biochemical and genetic mechanisms of homing.
  • Structural and functional studies of representative homing endonuclease families.

Related Experiment Videos

  • Comparative analysis with restriction endonucleases.

    • Main Results:

    • Homing endonucleases exhibit unique features, including flexible recognition of long DNA targets.
    • These enzymes minimize off-target cleavage in the host genome.
    • Recent studies provide insights into the biochemical, genetic, structural, and functional aspects of homing endonucleases.

    Conclusions:

    • Homing endonucleases are crucial for understanding gene transfer and mobile genetic elements.
    • Their unique DNA binding and cleavage mechanisms offer insights into enzyme evolution and function.
    • Further research on these endonucleases can advance gene editing technologies and our understanding of genome dynamics.