Suppression of both basal and antigen-induced lipid peroxidation in ring dove heterophils by melatonin

There have been several findings recently concerning melatonin as a free radical scavenger and general antioxidant. For instance, in bird heterophils we found that 100 microM of melatonin decreases superoxide anion levels and modulates superoxide dismutase activity. This paper sought to study the effect of melatonin upon induced oxidative damage in heterophils of the ring dove (Streptopelia risoria). The concentration of malonaldehyde (MDA) as an index of induced oxidative damage to lipid membranes was tested by colorimetric assay. A heterophil suspension was co-incubated with and without inert particles (latex beads) as material to be phagocytosed, both alone and in combination with 100 microM of melatonin. Measurements were made at the basal time (0 min), as well as at 15, 30, 45, and 60 min. Protein concentrations were determined by a standardized method using bovine serum albumin as standard. Results are expressed as nmol MDA/mg prot. Melatonin clearly reduced the production of MDA, an index of lipid peroxidation. It also annulled the enhancement of MDA levels produced by latex beads. Both effects were observed at all the times studied. In conclusion, our findings again show that the neurohormone melatonin could be useful as an effective pharmacological antioxidant.