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Extracellular matrixes influence alpha-smooth muscle actin expression in cultured porcine lens epithelial cells
1Keio University School of Medicine Department of Ophthalmology Tokyo, Japan. kurosaka@mc.med.keio.ac.jp
Purpose:
To determine whether extracellular matrix (ECM) influences the expression of alpha-smooth muscle actin (alpha-SMA), a marker for myofibroblasts, in cultured porcine lens epithelial cells (LECs).
Methods:
Porcine LECs were cultured for 6 days in an F-12 nutrient mixture supplemented with 5% fetal bovine serum on wells that were coated with laminin, fibronectin, type I collagen, or type IV collagen. LECs cultured on uncoated wells served as a control. Alpha-SMA was detected immunocytochemically with a mouse monoclonal antibody, and the ratio of the number of alpha-SMA-positive cells to the total number of cells, the P/T ratio, was calculated.
Results:
The P/T ratio of the LECs on the uncoated dishes was about 5%. LECs cultured on wells coated with laminin or type IV collagen significantly reduced the ratio, whereas fibronectin or type I collagen had no effect.
Conclusions:
The ECM influences alpha-SMA expression in cultured porcine LECs.