Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Expression of HBsAg by using various eukaryotic expression vectors].

B Gu1, H Ren

  • 1Chongqing Medical University.

Zhonghua Gan Zang Bing Za Zhi = Zhonghua Ganzangbing Zazhi = Chinese Journal of Hepatology
|September 17, 1999
PubMed
Summary

This study developed an efficient eukaryotic expression system for Hepatitis B surface antigen (HBsAg) and its variants. Different expression vectors showed varying efficiencies, establishing a foundation for HBsAg research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of topology on performance of pore networks in membrane filters.

Physical review. E·2026
Same author

Multimodality treatment maximizing outcome in spinal dural arteriovenous fistulae.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2025
Same author

Fractal dimension of non-Newtonian Hele-Shaw flow subject to Saffman-Taylor instability.

Physical review. E·2025
Same author

[Establishment of a rapid method for detection of influenza A/B virus' antigens].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2023
Same author

Preoperative prediction of microvascular invasion and perineural invasion in pancreatic ductal adenocarcinoma with <sup>18</sup>F-FDG PET/CT radiomics analysis.

Clinical radiology·2023
Same author

[A retrospective analysis of clinical characteristics and prognostic factors for 152 cases of <i>Staphylococcus aureus</i> bloodstream infection].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2023

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Context:

  • Hepatitis B virus (HBV) surface antigen (HBsAg) plays a crucial role in HBV infection and is a target for vaccines.
  • Developing efficient expression systems for HBsAg and its variants is essential for studying their biological characteristics and for therapeutic applications.

Purpose:

  • To establish and compare the efficiency of various eukaryotic expression systems for producing HBsAg and its variants.
  • To identify optimal expression vectors for stable and high-level production of recombinant HBsAg.

Summary:

  • A series of expression vectors were constructed by cloning the HBV S gene and its mutant genes into eukaryotic vectors (pMEP4, pCEP4, pLXSN, PXT1, pcDNA3).
  • These vectors were transfected into human hepatocellular carcinoma cells (HepG2), leading to the stable establishment of cell lines expressing recombinant HBsAg and its variants.

Related Experiment Videos

  • All tested vectors expressed HBsAg and its variants, but significant differences in transfection efficiency, expression levels, and stability were observed.

    • Impact:

    • The study successfully established eukaryotic expression systems for HBsAg and its variants, demonstrating varying efficiencies among different vectors.
    • This work provides a valuable platform for in-depth investigation of HBsAg variant biological properties.
    • The developed systems are crucial for advancing research in genetic immunization and genetic therapy strategies against Hepatitis B.