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[Abnormal expression of several oncogenes during hepatocarcinogenesis in WHV/myc transgenic mice]
1First Affiliated Hospital of Nanjing Medical University.
Objective:
To explore at cellular level the abnormal expression of related oncogenes during hepatocarcinogenesis in the WHV/myc transgenic mice.
Methods:
With specific molecular probes, in situ hybridization was used to detect the expression of c-myc transgene,IGF-II gene as well as c-jun, c-fos, and c-H-ras oncogenes in the liver sections from WHV/myc transgenic mice in different stages of the carcinogenic process.
Results:
The overexpression of c-myc transgene and IGF-II gene transcripts were detected in liver sections from the earlier neonatal transgenic mice. Then, the expression levels of these two genes were rapidly decreased and undetectable. At the time of the liver tumor development, the expression of c-myc transgene and IGF-II gene resumed. The c-jun, c-fos and c-H-ras genes expressed more intensely in the livers from preneoplastic WHV/myc mice than from normal mice.
Conclusion:
The overexpressions of c-myc transgene and IGF-II gene in the earlier neonatal stage and tumoral stage may be of importance for the development and maintenance of a transformed phenotype. During the prenoplastic "silent" period of c-myc transgene expression in WHV/myc transgenic mice, several oncogenes are overexpressed in the livers.