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Related Experiment Videos

CD14 expression and production by human hepatocytes.

G L Su1, K Dorko, S C Strom

  • 1Department of Medicine, University of Michigan, Ann Arbor 48109-0666, USA. gsu@umich-edu

Journal of Hepatology
|September 17, 1999
PubMed
Summary

Human hepatocytes produce CD14, a receptor for lipopolysaccharide (LPS). This finding suggests a novel role for the liver in LPS clearance, potentially impacting immune responses and sepsis treatment.

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Area of Science:

  • Immunology
  • Hepatology
  • Molecular Biology

Background:

  • CD14 acts as a lipopolysaccharide (LPS) receptor, existing in membrane-bound (mCD14) and soluble (sCD14) forms.
  • Monocytes are known producers of sCD14, but the liver's role remains uncertain.
  • The liver is crucial for clearing LPS from circulation.

Purpose of the Study:

  • To investigate the capacity of human hepatocytes to produce CD14.
  • To determine if hepatocytes contribute to the circulating pool of sCD14.
  • To explore a potential mechanism for hepatic LPS clearance.

Main Methods:

  • Isolation of primary human hepatocytes via collagenase perfusion.
  • Detection of CD14 mRNA using Northern blot analysis.
  • Confirmation of CD14 protein expression through Western blot and immunohistochemistry.

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Main Results:

  • Human hepatocytes express CD14 mRNA and protein.
  • A liver epithelial cell line (AKN-1) also demonstrated CD14 production.
  • Hepatocyte-derived CD14 appears slightly larger than that from monocytic cell lines.

Conclusions:

  • This study provides the first evidence of CD14 production by human hepatocytes.
  • Hepatocytes represent a potential source of sCD14.
  • Hepatic CD14 production may contribute to LPS clearance and immune regulation.