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Template secondary structure promotes polymerase jumping during PCR amplification.
V K Viswanathan1, K Krcmarik, N P Cianciotto
1Northwestern University, Chicago, IL, USA.
Biotechniques
|September 18, 1999
Summary
Investigating Legionella pneumophila mutants revealed unexpected polymerase chain reaction (PCR) product sizes. This anomaly in mini-transposon (Tn10) insertion sites resulted from secondary DNA structures, not insertion size.
Area of Science:
- Molecular Biology
- Genetics
- Microbiology