Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tuberculin skin testing: determinants and reaction.

L Y Lao1, T De Guia

  • 1Philippine Heart Center, Section of Pulmonary and Critical Care Medicine, Quezon City, Philippines. phcpulmo@epic.net

Respirology (Carlton, Vic.)
|September 18, 1999
PubMed
Summary

Close contact with active tuberculosis (TB) cases significantly increases Mantoux purified protein derivative (PPD) skin test reactivity in schoolchildren. Repeat bacille Calmette-Guérin (BCG) vaccination and BCG scars can complicate TB diagnosis but do not alter PPD reactivity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DRAM-3 modulates autophagy and promotes cell survival in the absence of glucose.

Cell death and differentiation·2017
Same author

DRAM-3 modulates autophagy and promotes cell survival in the absence of glucose.

Cell death and differentiation·2015
Same author

Asthma programmes in diverse regions of the world: challenges, successes and lessons learnt.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2011
See all related articles

Area of Science:

  • Immunology
  • Public Health
  • Pediatrics

Background:

  • Tuberculosis (TB) diagnosis in children can be challenging, particularly when considering factors like prior bacille Calmette-Guérin (BCG) vaccination.
  • Mantoux purified protein derivative (PPD) skin testing is a key tool for assessing TB infection, but its reactivity can be influenced by various factors.

Purpose of the Study:

  • To evaluate the impact of repeat bacille Calmette-Guérin (BCG) vaccination, nutritional status, BCG scar presence, and sputum acid-fast bacilli (AFB) positivity on PPD skin reactivity in schoolchildren with and without recent TB family contact.
  • To determine the significance of close contact with smear-positive TB cases on PPD skin test conversion.

Main Methods:

  • A prospective case-control study involving schoolchildren grouped by exposure to AFB smear-positive TB family contacts (Group I) versus controls (Group II).

Related Experiment Videos

  • Mantoux PPD skin testing was administered, and induration size was measured.
  • Nutritional status was assessed using percentile-based weight categories.
  • Data on repeat BCG vaccinations, BCG scar presence, and AFB sputum positivity in adult TB cases were collected.

    • Main Results:

    • Group I (TB contact) showed significantly larger PPD induration (13.00 +/- 11.29 mm) compared to Group II (4.52 +/- 6.20 mm; P = 0.000).
    • Repeat BCG vaccination correlated with increased PPD induration size, particularly in Group I (P = 0.048).
    • Children with BCG scars in Group I had significantly greater PPD induration than Group II (14.14 +/- 11.23 vs 5.05 +/- 6.24 mm; P = 0.000).
    • Nutritional status and AFB positivity of adult TB cases did not significantly affect PPD reactivity.

    Conclusions:

    • Close contact with smear-positive TB individuals is a critical factor for PPD skin test conversion in schoolchildren.
    • While repeat BCG vaccination, malnutrition, and BCG scars may complicate TB diagnosis, they do not inherently alter PPD reactivity.
    • Thorough clinical evaluation is essential for accurate TB diagnosis, especially in the presence of confounding factors.