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Related Experiment Videos

Complement activation by PEO-grafted glass surfaces.

A Kidane1, K Park

  • 1Purdue University, School of Pharmacy, West Lafayette, Indiana 47907, USA.

Journal of Biomedical Materials Research
|September 22, 1999
PubMed
Summary

Pluronic F108 (PF108) grafted to surfaces activates the complement system, unlike poly(ethylene oxide) (PEO). This complement activation is linked to PF108

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Area of Science:

  • Biomaterials Science
  • Immunology
  • Surface Chemistry

Background:

  • The human body's complement system activates upon contact with foreign materials.
  • Poly(ethylene oxide) (PEO) is widely used to coat biomaterials, reducing protein adsorption and cell adhesion.
  • The complement-activating potential of PEO-grafted surfaces has not been previously investigated.

Purpose of the Study:

  • To investigate complement activation by poly(ethylene oxide) (PEO) chains grafted onto glass surfaces.
  • To determine if different PEO-based polymers (homopolymer, Pluronic F108, and a triblock copolymer) elicit varying complement responses.
  • To understand the relationship between surface polymer concentration and complement activation.

Main Methods:

  • Grafting of PEO homopolymer, Pluronic F108 (PF108), and PEO-polybutadiene-PEO triblock copolymer (COP5000) onto trichlorovinylsilane-treated glass (TCVS-glass) via gamma-irradiation.
  • Quantification of complement activation by measuring plasma C3a levels.
  • Analysis of the dose-dependent relationship between PF108 surface concentration and C3a production.
  • Kinetic studies of C3a generation and assessment of complement activation upon repeated plasma exposure.

Main Results:

  • Only Pluronic F108 (PF108)-grafted surfaces significantly increased complement activation compared to controls.
  • Complement C3a production on PF108-grafted glass showed a linear dependence on surface PF108 concentration.
  • C3a levels increased from 46 ng/mL to 316 ng/mL as PF108 surface concentration rose from 0 to 0.25 µg/cm².
  • Complement activation by PF108-grafted surfaces followed a "single-hit" mechanism, with reduced C3a generation upon repeated plasma exposure.
  • PEO homopolymer did not activate complement in bulk solution, suggesting poly(propylene oxide) units in PF108 are responsible for activation.

Conclusions:

  • Pluronic F108 (PF108), not PEO homopolymer, induces complement activation when grafted to surfaces.
  • The presence of poly(propylene oxide) units within PF108 is likely responsible for complement activation.
  • Surface concentration of PF108 directly influences the degree of complement activation.
  • Block copolymers containing PEO require careful evaluation of their non-PEO segments for complement-activating properties.

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