Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitochondria and cell death. Mechanistic aspects and methodological issues.

P Bernardi1, L Scorrano, R Colonna

  • 1CNR Unit for the Study of Biomembranes, Department of Biomedical Sciences, University of Padova, Italy. bernardi@civ.bio.unipd.it

European Journal of Biochemistry
|September 22, 1999
PubMed
Summary

Mitochondria play a crucial role in programmed cell death (apoptosis) by releasing key proteins. Understanding their complex involvement requires analyzing mitochondrial physiology and evaluating in situ monitoring methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The innate immune receptor NLRX1 is a novel required modulator for mPTP opening: implications for cardioprotection.

Basic research in cardiology·2025
Same author

Human neural stem cells derived from fetal human brain communicate with each other and rescue ischemic neuronal cells through tunneling nanotubes.

Cell death & disease·2024
Same author

Homogeneity assessment of the SuperCam calibration targets onboard rover perseverance.

Analytica chimica acta·2022
Same author

Author Correction: Impact of sacubitril/valsartan on implantable defibrillator eligibility in heart failure: a real-world experience.

European review for medical and pharmacological sciences·2022
Same author

Impact of sacubitril/valsartan on implantable defibrillator eligibility in heart failure: a real-world experience.

European review for medical and pharmacological sciences·2021
Same author

SuperCam Calibration Targets: Design and Development.

Space science reviews·2020

Area of Science:

  • Cell Biology
  • Biochemistry
  • Physiology

Background:

  • Mitochondria's role in cell death extends beyond ATP production.
  • Mitochondria release proteins like cytochrome c, crucial for apoptosis.
  • The exact role of mitochondria in cell death (cause vs. consequence) is complex and debated.

Purpose of the Study:

  • To elucidate the mechanistic aspects of mitochondrial involvement in cell death.
  • To emphasize the role of mitochondrial permeability transition and its effects.
  • To critically evaluate methods for monitoring mitochondrial function in situ.

Main Methods:

  • Analysis of mitochondrial physiology.
  • Focus on mitochondrial permeability transition.
  • Evaluation of in situ monitoring techniques.

Related Experiment Videos

Main Results:

  • Mitochondrial responses depend on stimulus type, intensity, and perturbed function.
  • Mitochondrial permeability transition leads to swelling, cytochrome c release, and respiratory inhibition.
  • Integration of mitochondrial responses is complex and difficult to dissect.

Conclusions:

  • Mitochondria are central players in apoptosis through protein release.
  • Mitochondrial permeability transition is a key event in cell death.
  • Careful evaluation of in situ methods is necessary for accurate assessment of mitochondrial function in cell death.