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Ca2+-dependent activity of human DNase I and its hyperactive variants

C Q Pan1, R A Lazarus

  • 1Department of Protein Engineering, Genentech, Inc., South San Francisco, California 94080, USA.

Protein Science : a Publication of the Protein Society
|September 24, 1999
PubMed
Summary

Researchers engineered hyperactive deoxyribonuclease I (DNase I) variants with distinct calcium (Ca2+) dependencies. Class B variants, featuring positive charge substitutions, show reduced Ca2+ dependence, offering insights into enzyme activity regulation.

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