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Chemokine production by human chondrocytes
L Pulsatelli1, P Dolzani, A Piacentini
1Laboratorio Immunologia e Genetica, Istituto Codivilla Putti-IOR; Bologna, Italy.
The Journal of Rheumatology
|September 24, 1999
Summary
Chondrocytes produce various chemokines, including IL-8 and MCP-1, in joint diseases like RA and OA. Proinflammatory cytokines significantly increase production, while anti-inflammatory factors have minimal impact.
Area of Science:
- Rheumatology
- Immunology
- Cell Biology
Background:
- Chondrocytes, the primary cells of articular cartilage, play a role in joint homeostasis.
- The production of chemokines by chondrocytes in inflammatory joint diseases like rheumatoid arthritis (RA) and osteoarthritis (OA) is not fully understood.
Purpose of the Study:
- To investigate the role of chondrocytes in producing CXC chemokines (IL-8, GRO-alpha) and CC chemokines (MCP-1, MIP-1alpha, RANTES).
- To compare chemokine production in chondrocytes from patients with RA, OA, and healthy subjects (PT).
- To assess the influence of proinflammatory and anti-inflammatory mediators on chondrocyte chemokine production.
Main Methods:
- Articular cartilage was obtained from patients with OA, RA, and trauma (PT).
- Chondrocytes were isolated and cultured in vitro.
- Chemokine production was measured using ELISA after stimulation with IL-1beta, TNF-alpha, TGF-beta1, and IL-10.
Main Results:
- Chondrocytes produce IL-8, GRO-alpha, MCP-1, MIP-1alpha, and RANTES.
- IL-1beta was a more potent stimulus than TNF-alpha for most chemokines.
- No significant differences in chemokine production were observed between OA, RA, and PT chondrocytes.
- TGF-beta1 modulated IL-1beta-induced chemokine production, while IL-10 had no significant effect.
Conclusions:
- Chondrocytes are a source of various chemokines in the joint.
- Proinflammatory cytokines strongly induce chondrocyte chemokine production.
- Chondrocyte-derived chemokines may contribute to the pathogenesis and persistence of joint diseases.