Behavioral analysis of PTZ-kindled rats after acute and chronic ethanol treatments

The present study was designed to examine the response of PTZ-kindled and saline-injected animals to both acute and chronic ethanol treatment. Acute injection of ethanol (3.0 g/kg; IP) resulted in a rapid onset of loss of righting reflex (LORR) in both PTZ-kindled and saline-injected animals. However, the PTZ-kindled animals recovered from LORR significantly more quickly than control animals. Using a tilt-plane test as a measure of motor incoordination, the PTZ-kindled animals had significantly less motor incoordination compared to controls. Blood alcohol levels (BAL) were not significantly different between the groups. We also compared the degree of tolerance and dependence in chronic ethanol-treated, PTZ-kindled, and control animals. PTZ-kindled, saline-injected and naive control animals were chronically treated with ethanol vapor. The PTZ-kindled group tolerated high vapor concentrations (in terms of food consumed/rat) and, at the end of the treatment, displayed intoxication characteristics different from those of the control groups despite having similar blood alcohol levels. The PTZ-kindled group also displayed withdrawal behavior that was similar to a group of ethanol-treated animals that had experienced a prior cycle of dependency and withdrawal. These data show many intriguing similarities between animals that are PTZ-kindled and chronically treated with ethanol and suggest the use of PTZ-kindled animals as a model for alcohol withdrawal kindling.