Tryptamine, serotonin and catecholamines: an immunocytochemical study in the central nervous system

Tryptamine, a serotonin-related indolamine, could be involved in the modulation of catecholaminergic and serotoninergic systems interaction. Despite previous reports on this topic, the morphological relationship among these systems is not well described. We studied the interaction among serotoninergic and catecholaminergic with tryptaminergic systems by double immunostaining at the level of light microscopy. Mesencephalic rat brain sections treated according to the Schiff quenching method were double immunostained using peroxidase and fluorescein labeled antibodies. Primary antibodies to anti-tryptophan hydroxylase (TrpOH), anit-tyrosine hydroxylase (TH) and anti-tryptamine (T) were used to demonstrate serotoninergic, catecholaminergic and tryptaminergic neurons respectively. A morphometric study was performed in order to analyze the different morphological characteristics of each system. The results showed that (i) T+ and TrpOH+ neurons are localized in the same areas but their morphology is significantly different. Moreover morphometric parameters of T+ neurons were significantly different from those TrpOH+ or TH+ neurons; (ii) The number of TrpOH+ neurons was larger than T+ neurons; (iii) T+ neurons were dominant in the lateral dorsal raphe nucleus. TrpOH+ neurons were more numerous in the central area of the dorsal raphe nucleus; (iv) Coexpression of TrpOH and T was demonstrated in the somata of dorsal raphe nucleus neurons; (v) TrpOH+ neurons from raphe nuclei and TH+ neurons from substantia nigra are contacted by T+ fibres. The present morphological evidence supports a functional relationship among these three aminergic systems.