Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thrombopoietin.

W S Alexander1

  • 1Walter and Eliza Hall Institute for Medical Research, Royal Melbourne Hospital, Victoria, Australia. alexander_w@wehi.edu.au

Growth Factors (Chur, Switzerland)
|September 25, 1999
PubMed
Summary
This summary is machine-generated.

Thrombopoietin (TPO) regulates platelet production by binding its receptor c-Mpl, influencing megakaryocytes and stem cells. This signaling pathway is key for maintaining platelet levels and shows therapeutic potential for thrombocytopenia.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulation of platelet lifespan in the presence and absence of thrombopoietin signaling.

Journal of thrombosis and haemostasis : JTH·2016
Same author

BET inhibition represses miR17-92 to drive BIM-initiated apoptosis of normal and transformed hematopoietic cells.

Leukemia·2016
Same author

BCL-2 is dispensable for thrombopoiesis and platelet survival.

Cell death & disease·2015
Same author

Hhex regulates Kit to promote radioresistance of self-renewing thymocytes in Lmo2-transgenic mice.

Leukemia·2014
Same author

The Myb-p300-CREB axis modulates intestine homeostasis, radiosensitivity and tumorigenesis.

Cell death & disease·2013
Same author

SOCS2 is dispensable for BCR/ABL1-induced chronic myeloid leukemia-like disease and for normal hematopoietic stem cell function.

Leukemia·2012
Same journal

The role of ERK signaling pathway in pituitary adenomas: from molecular mechanisms to targeted therapy.

Growth factors (Chur, Switzerland)·2026
Same journal

Role of soluble EGFR, Fas, and Fas ligand in pelvic pain severity and their association with endometriosis in infertile woman: a case-control study.

Growth factors (Chur, Switzerland)·2026
Same journal

ErbB receptor stimulation is required for mouse Colon adenoma organoids to form crypts.

Growth factors (Chur, Switzerland)·2026
Same journal

Chorioallantoic membrane assay demonstrating pro-angiogenic properties of BRAF inhibitor: a tool to treat chronic wounds.

Growth factors (Chur, Switzerland)·2026
Same journal

Extracellular matrix biomarkers and transforming growth factor-β in hypertrophic cardiomyopathy: interaction between biomarkers.

Growth factors (Chur, Switzerland)·2026
Same journal

Growth factors loaded chitosan scaffolds as bioactive biomimetic multifunctional platforms in periodontal tissue engineering: a review.

Growth factors (Chur, Switzerland)·2025
See all related articles

Area of Science:

  • Hematology
  • Molecular Biology
  • Cell Signaling

Background:

  • Thrombopoietin (TPO) was initially identified by its ability to stimulate platelet production.
  • The molecular cloning of TPO enabled detailed characterization of its biological functions.
  • TPO exerts its effects by binding to its specific receptor, c-Mpl, on target cells.

Purpose of the Study:

  • To characterize the biological activities of TPO in vitro and in vivo.
  • To elucidate the signaling pathways activated by TPO/c-Mpl interaction.
  • To investigate the role of TPO in regulating megakaryopoiesis and stem cell function.

Main Methods:

  • Molecular cloning and characterization of TPO.
  • In vitro and in vivo studies of TPO activity.

Related Experiment Videos

  • Gene targeting studies to investigate the TPO/c-Mpl signaling system.
  • Preclinical studies in animal models and clinical trials of c-Mpl ligands.

    • Main Results:

    • TPO binding to c-Mpl induces receptor dimerization and activates intracellular signaling pathways (JAK-STAT, Ras).
    • TPO is a lineage-dominant cytokine that stimulates megakaryocyte production and maturation.
    • TPO/c-Mpl signaling is the primary regulator of steady-state megakaryocyte and platelet production.
    • TPO plays a significant role in regulating hematopoietic stem cells.
    • c-Mpl ligands have demonstrated efficacy in elevating platelet counts in clinical trials.

    Conclusions:

    • The TPO/c-Mpl signaling system is crucial for platelet production and hematopoietic stem cell regulation.
    • TPO-based therapeutics show promise for treating thrombocytopenia, particularly that associated with chemotherapy and radiotherapy.