Backbone dynamics of a winged helix protein and its DNA complex at different temperatures: changes of internal
1Department of Biochemistry and Molecular Biology, College of Medicine, M/C 536, University of Illinois at Chicago, 1819 West Polk Street, Chicago, IL 60612, USA.
Abstract:
The dynamic properties of a winged helix protein, Genesis, and its DNA complex at different temperatures were studied. Due to the complexity of motions, the commonly used model-free formalism could not be used to reflect the dynamic properties. The reduced spectral density function mapping approach was proven to be a useful tool to describe the overall and internal motion of molecules on the picosecond to nanosecond time-scale, and conformational exchanges on the microsecond to millisecond time-scale. The local motions in DNA-free Genesis showed strong temperature dependence and the backbone dynamics of each secondary structural element responds to the temperature change differently, while the Genesis-DNA complex showed more stability with changing the temperatures. Furthermore, each DNA contact sequence of Genesis showed distinct dynamic perturbation after Genesis binds to DNA.
Related Concept Videos
Single-Strand DNA Binding Proteins
DNA Helicases
The DNA Helix
The DNA Helix
Nucleosome Remodeling
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
DNA as a Genetic Template


