Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Metal binding to the HIV nucleocapsid peptide.

G McLendon1, H Hull, K Larkin

  • 1Department of Chemistry, Princeton University, NJ 08544, USA.

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
|September 28, 1999
PubMed
Summary

This study quantifies cobalt (Co(II)) and zinc (Zn(II)) binding to the HIV-1 nucleocapsid protein

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Individual differences in medical radiation exposure in CT dosimetry based on age and BMI.

Annals of the ICRP·2026
Same author

Corrigendum to "Fruquintinib in refractory metastatic colorectal cancer: a multicenter real-world study": [ESMO Open 9 (2024) 103702].

ESMO open·2025
Same author

Fruquintinib in refractory metastatic colorectal cancer: a multicenter real-world study.

ESMO open·2024
Same author

[Application of three risk assessment methods to noise risk assessment in an automobile foundry enterprise].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2022
Same author

Λ_{c}^{+} Production and Baryon-to-Meson Ratios in pp and p-Pb Collisions at sqrt[s_{NN}]=5.02  TeV at the LHC.

Physical review letters·2021
Same author

Age-related alterations to working memory and to pyramidal neurons in the prefrontal cortex of rhesus monkeys begin in early middle-age and are partially ameliorated by dietary curcumin.

Neurobiology of aging·2021

Area of Science:

  • Biochemistry
  • Structural Biology
  • Virology

Background:

  • The HIV-1 nucleocapsid protein (NCp) is essential for viral replication.
  • NCp contains metal-binding domains that are crucial for its function.
  • Understanding metal ion interactions with NCp is key to developing antiviral therapies.

Purpose of the Study:

  • To determine the binding affinities and thermodynamics of Co(II) and Zn(II) to the HIV-1 NCp N-terminal metal-binding domain (residues 1-18).
  • To compare these findings with existing data for related systems, including the full-length protein.

Main Methods:

  • Co(II) and Zn(II) binding constants were measured using competition titration.
  • Co(II) binding was monitored via visible absorbance spectroscopy.
  • Enthalpies of binding were determined using isothermal titration calorimetry.

Main Results:

  • Quantified Co(II) and Zn(II) binding constants for the HIV-1 NCp N-terminal domain.
  • Measured the enthalpies of metal ion binding.
  • Provided thermodynamic data for metal coordination in this specific NCp domain.

Conclusions:

  • The study provides crucial thermodynamic parameters for Co(II) and Zn(II) binding to a key domain of the HIV-1 nucleocapsid protein.
  • These data contribute to understanding the role of metal ions in HIV-1 NCp structure and function.
  • Findings can inform the design of novel antiviral agents targeting NCp metal binding.

Related Experiment Videos