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Cloning, sequence analysis, and expression of ansB from Pseudomonas fluorescens, encoding periplasmic
A Hüser1, U Klöppner, K H Röhm
1Philipps-Universität, Institut für Physiologische Chemie, Marburg, Germany.
FEMS Microbiology Letters
|September 28, 1999
Abstract:
A gene (ansB) encoding a class II glutaminase/asparaginase has been cloned from Pseudomonas fluorescens and characterized by DNA sequencing, promoter analysis and heterologous expression in Escherichia coli. We show that ansB is monocistronic and depends on the alternate sigma factor sigma 54 for expression. A second open reading frame located downstream of ansB is highly homologous to a number of bacterial genes that encode secreted endonucleases of unknown function.