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Differentiation of Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus by polymerase chain

L J Ward1, M J Timmins

  • 1New Zealand Dairy Research Institute, Palmerston North, New Zealand. lawrence.ward@nzdri.org.nz

Letters in Applied Microbiology
|September 28, 1999
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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