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Analysis of spiramycin by capillary electrophoresis
R González-Hernández1, Y M Li, A Van Schepdael
1Laboratorio de Análisis, Centro de Química Farmacéutica, Playa, La Habana, Cuba.
Electrophoresis
|September 28, 1999
Summary
A new capillary electrophoresis method accurately determines spiramycin I alongside related substances. This validated analytical technique offers high precision and sensitivity for spiramycin analysis.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Spiramycin I is an important antibiotic.
- Accurate determination of spiramycin I in the presence of related substances is crucial for quality control.
Purpose of the Study:
- To develop and validate a capillary electrophoresis (CE) method for spiramycin I determination.
- To achieve good separation of spiramycin I from its related substances.
Main Methods:
- Capillary electrophoresis using a fused-silica capillary.
- Phosphate buffer with cetyltrimethylammonium bromide (CTAB) and sodium cholate.
- Optimized voltage, temperature, and buffer conditions.
Main Results:
- Achieved separation of spiramycin I and related substances within 15 minutes.
- Demonstrated method linearity (r = 0.9992) and precision (RSD = 1.3%).
- Established low limits of detection (7 pg) and quantitation (22 pg).
Conclusions:
- The developed CE method is selective, robust, linear, and precise.
- This validated method is suitable for the determination of spiramycin I in the presence of related substances.