Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Separating DNA sequencing fragments without a sieving matrix.

H Ren1, A E Karger, F Oaks

  • 1Biology Department, University of Ottawa, ON, Canada.

Electrophoresis
|September 28, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbiota changes in rumen and milk corresponding to dietary protein intake in transition dairy cows.

Journal of dairy science·2026
Same author

Field-driven polyelectrolyte-polymer collisions in nanochannels.

The Journal of chemical physics·2024
Same author

Estimating the asymptotic characteristic time scales for diffusion-controlled drug release systems using partially sampled data.

International journal of pharmaceutics·2023
Same author

Diffusivity interfaces in lattice Monte Carlo simulations: Modeling inhomogeneous delivery and release systems.

Physical review. E·2022
Same author

Chemotherapy for De Novo Gastric Adenocarcinoma After Deceased Orthotopic Liver Transplantation: A Case Report.

Transplantation proceedings·2017
Same author

[Study of disease burden of chronic hepatitis B and C patients in Shanghai based on Bronfenbrenner' s ecological systems theory: a community-based survey].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2017
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
Same journal

CMOS Electrokinetic Systems and Fabrication Approaches for On-CMOS 3D Electrodes.

Electrophoresis·2026
See all related articles

End-labeled free-flow electrophoresis (ELFSE) can sequence single-stranded DNA (ssDNA) without sieving matrices. This method offers a rapid, matrix-free alternative for DNA sequencing in capillaries and microchips.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Free-flow capillary electrophoresis (CE) has shown promise for DNA fragment separation.
  • Previous work demonstrated free-flow separation of double-stranded DNA (dsDNA) using streptavidin labels.
  • The theoretical possibility of separating labeled DNA fragments via free-flow CE was previously established.

Purpose of the Study:

  • To investigate the potential of end-labeled free-flow electrophoresis (ELFSE) for single-stranded DNA (ssDNA) sequencing.
  • To determine the feasibility of matrix-free ssDNA sequencing using ELFSE.
  • To assess the speed and efficiency of ELFSE for DNA sequencing.

Main Methods:

  • End-labeling of ssDNA fragments with fractionated streptavidin at the 5'-end.

Related Experiment Videos

  • Separation of labeled ssDNA fragments using free-flow electrophoresis without a sieving matrix.
  • Analysis of separated fragments to determine sequencing capability.

    • Main Results:

    • Successfully sequenced the first 100 bases of ssDNA using ELFSE without a sieving matrix.
    • Achieved separation and sequencing in just 18 minutes.
    • Demonstrated that coated capillaries are not required for this method.
    • Identified sample injection, analyte-wall interactions, and thermal diffusion as current limiting factors.

    Conclusions:

    • End-labeled free-flow electrophoresis (ELFSE) is a viable method for sequencing single-stranded DNA (ssDNA).
    • ELFSE offers a rapid and matrix-free approach for DNA sequencing, potentially outperforming current polymer-based methods.
    • Further optimization of conditions could enable sequencing of several hundred bases in under 30 minutes, making it attractive for capillary and microchip applications.