Related Experiment Video
Updated: Oct 19, 2026

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
Published on: December 21, 2019
The characterization and phylogenetic relationship of the Trichoplusia ni single capsid nuclear polyhedrosis virus
1University of the Western Cape, Faculty of Science, Department of Microbiology, Bellville, South Africa. bertie@mbiol.uwc.ac.za
Abstract:
The polyhedrin gene (polh) was identified from the Trichoplusia ni (Tni) single capsid nuclear polyhedrosis virus (SNPV). An EcoRI fragment containing the truncated polyhedrin gene was detected by hybridization with an AcMNPV expression vector probe; the remaining portion of the gene was amplified by reverse PCR. An open reading frame (ORF) of 741 nucleotides (nt), encoding a putative protein of 246 amino acids (a.a) with Mr 28,780 Da was identified. The 5'-noncoding region contained the putative late (TAAG) transcription initiation motif. The 3' end, downstream of the translation stop codon, lacked an obvious putative poly (A) signal. Nucleotide and amino acid homology are greater than 80% to that of Mamestra brassicae polyhedrin sequences. Results suggest that T. niSNPV is a member of the group II nuclear polyhedrosis viruses.
Related Concept Videos
Subviral Agents
Inhibitors Of Virion Release
Viral Structure
Inhibitors of Virion Maturation and Assembly
Size and Structure of Viral Genomes
Polytene Chromosomes
