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Published on: July 21, 2014
A negative regulatory element in a prespore-specific promoter of dictyostelium discoideum(1)
1Department of Biochemistry, University of Oxford, South Parks Road, Oxford, UK.
Abstract:
We previously isolated several 'promoter-trap' transformants in which insertion of a promoterless beta-galactosidase gene into the genome caused expression of beta-galactosidase in specific cell types. The upstream flanking region was rescued from one transformant specifically expressing beta-galactosidase in prespore cells. We sequenced the promoter of the gene that is fused in-frame with lacZ and characterised a negative element that inhibits expression in pstO cells (a subtype of prestalk cells). Gel-retardation assays show that a developmentally regulated factor(s) recognises and binds to this element.
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