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The matrix protein gene sequence analysis reveals close relationship between peste des petits ruminants virus (PPRV)
1Département d'Elevage et de Médecine Vétérinaire, Cirad-emvt, Campus International de Baillarguet, 34032 Montellier, BP 5035, Montpellier, France.
Abstract:
The gene encoding the matrix protein of peste des petits ruminants virus (PPRV) has been cloned and its nucleotide sequence determined. This gene is 1466 nucleotides long and contains an open reading frame (ORF) capable of encoding a basic protein of 335 amino acid residues with a predicted molecular weight of 38,057 Da. This ORF starts at position 33-35 and ends with the codon TAA at position 1038-1040 thus leaving a long untranslated region (426 nucleotides) at the 3' end of the messenger RNA. This fragment is very G/C rich (68.5%) and in contrast to the ORF region appears to be least conserved in the M gene sequence of the morbilliviruses. A comparison of the PPRV M protein with those of other viruses in the group confirms the previously noted high degree of conservation for this protein sequence. The percent of identity within the group ranges from 76.7 to 86.9%, the highest being with the dolphin morbillivirus matrix protein.
Insights
The matrix protein gene of peste des petits ruminants virus (PPRV) was sequenced, revealing a 335-amino acid protein. This PPRV matrix protein shows high conservation with other morbilliviruses.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Peste des petits ruminants virus (PPRV) is a significant pathogen affecting small ruminants.
- The matrix (M) protein is a key structural component of morbilliviruses, essential for viral assembly and release.
Purpose of the Study:
- To clone and determine the nucleotide sequence of the PPRV matrix protein gene.
- To analyze the sequence characteristics of the PPRV M gene and its encoded protein.
- To compare the PPRV M protein sequence with those of other morbilliviruses.
Main Methods:
- Gene cloning and nucleotide sequencing of the PPRV M gene.
- Open reading frame (ORF) analysis to predict protein sequence and molecular weight.
- Sequence alignment and comparison with homologous genes from other morbilliviruses.
Main Results:
- The PPRV M gene is 1466 nucleotides long, encoding a 335-amino acid protein (38,057 Da).
- A long, G/C-rich 3' untranslated region (426 nucleotides) was identified, showing low conservation.
- High sequence identity (76.7-86.9%) was observed between PPRV M protein and other morbillivirus M proteins, particularly dolphin morbillivirus.
Conclusions:
- The M gene sequence provides valuable information for understanding PPRV molecular biology.
- The high conservation of the M protein suggests its critical role in morbillivirus structure and function.
- Comparative analysis aids in understanding morbillivirus evolution and relationships.