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Selection of human anti-HAV McAb from a phage antibody library
1Department of Microbiology, Third Military Medical University, Chongqing, China.
Chinese Journal of Biotechnology
|September 30, 1999
Abstract:
The phage displaying antibody fragments were subjected to three rounds of panning with hepatitis A virus (HAV) antigen in solid phase. The eluted phage was enriched nearly 100 fold, and the percentage of recombinant clones increased from 25% to 100% after three rounds of panning. The HAV antigen-specific monoclonal antibody (McAb) was screened by sandwich ELISA, and the specificity of these antibodies was further confirmed by competitive inhibition ELISA.