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Updated: Oct 19, 2026

07:15
Anaerobic Growth and Maintenance of Mammalian Cell Lines
Published on: July 21, 2018
Tetrahymena as a laboratory organism: useful strains, cell culture, and cell line maintenance
E Orias1, E P Hamilton, J D Orias
1Department of Molecular, Cellular and Developmental Biology, University of California at Santa Barbara 93106, USA.
Methods in Cell Biology
|September 30, 1999
Abstract
No abstract available in PubMed .
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Cell Lines
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

