Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HSF3 is a major heat shock responsive factor duringchicken embryonic development.

Y Kawazoe1, M Tanabe, N Sasai

  • 1Department of Molecular and Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Japan.

European Journal of Biochemistry
|October 3, 1999
PubMed
Summary

Heat shock transcription factors (HSFs) regulate heat shock genes. In chickens, HSF3 is constantly expressed and becomes dominant, suggesting it plays a key role alongside HSF1 in heat stress responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High levels of DNA polymerase β mRNA corresponding with the high activity in Graves' thyroid tissue.

Journal of endocrinological investigation·2016
Same author

Heat shock transcription factor 1-associated expression of slow myosin heavy chain in mouse soleus muscle in response to unloading with or without reloading.

Acta physiologica (Oxford, England)·2016
Same author

Influenza 2014-2015 among pregnant Japanese women: primiparous vs multiparous women.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2016
Same author

Deficiency of heat shock transcription factor 1 suppresses heat stress-associated increase in slow soleus muscle mass of mice.

Acta physiologica (Oxford, England)·2015
Same author

Vaccination during the 2013-2014 influenza season in pregnant Japanese women.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2014
Same author

A grasping forceps with a triaxial MEMS tactile sensor for quantification of stresses on organs.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2013

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Heat shock genes in vertebrates are primarily regulated by four heat shock transcription factors (HSFs).
  • Avian cells utilize at least three HSFs (HSF1, HSF2, and HSF3), with distinct activation patterns under heat stress.
  • HSF1 responds to mild heat shock, HSF3 to severe heat shock, and HSF2 is linked to developmental functions.

Purpose of the Study:

  • To investigate the temporal and spatial expression patterns of HSF1, HSF2, and HSF3 during chicken development.
  • To determine the relative contributions of different HSFs to heat shock gene regulation in avian tissues.

Main Methods:

  • Analysis of HSF expression levels across various chicken tissues during development.
  • Utilizing Northern blotting to assess the magnitude of heat shock response.

Related Experiment Videos

Main Results:

  • HSF3 exhibited constant expression across tissues throughout chicken development.
  • HSF1 expression was initially high and decreased in a tissue-specific manner.
  • HSF3 emerged as the dominant heat-responsive factor, and its expression did not always correlate with the heat shock response magnitude, suggesting a role for HSF3.

Conclusions:

  • HSF3 is a consistently expressed and dominant heat-responsive factor in chickens.
  • Both HSF1 and HSF3 likely mediate stress signals for heat shock gene expression.
  • Ubiquitous expression of HSF1, HSF2, and HSF3 during early embryogenesis suggests their involvement in developmental processes.