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07:06
Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
Multiple pathways for establishment of poliovirus infection
1Department of Microbiology, The Institute of Medical Science, The University of Tokyo, Japan.
Virus Research
|October 3, 1999
Summary
Poliovirus (PV) infection relies on specific interactions with the poliovirus receptor (PVR) for enhanced cell entry. In vivo studies show intact PV travels the sciatic nerve, with uncoating occurring after transport.
Area of Science:
- Virology
- Cell Biology
- Neuroscience
Background:
- Poliovirus (PV) utilizes the poliovirus receptor (PVR) for cell entry, involving virus binding and conformational changes.
- Understanding early PV infection mechanisms is crucial for developing antiviral strategies.
Purpose of the Study:
- To investigate the early infection process of Poliovirus using in vitro and in vivo models.
- To elucidate the role of PVR in PV infectivity and transport.
Main Methods:
- Established an in vitro PV infection system using mouse L cells expressing Fc gamma receptor molecules.
- Utilized PVR-IgG2a chimera for enhanced PV infection studies.
- Employed intramuscular inoculation of PV in PVR transgenic (Tg) mice to study in vivo neural pathway infection.
Main Results:
- Mouse anti-PV monoclonal antibodies (mAbs) mediated inefficient PV infection, which was enhanced by PVR-IgG2a.
- PVR-IgG2a induced a conformational change in PV virions (160S to 135S).
- In Tg mice, intact 160S PV particles were found in the sciatic nerve after intramuscular inoculation, indicating PVR-dependent neural transport.
Conclusions:
- Specific PVR-PV interactions beyond simple binding enhance PV infectivity in cultured cells.
- PV uncoating occurs after retrograde axonal transport through the sciatic nerve.
- Poliovirus infection can be established through multiple pathways.
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