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A polymerase chain reaction protocol for the detection of various geographical isolates of white spot virus
L M Tapay1, E C Nadala, P C Loh
1National Institute of Molecular Biology and Biotechnology, University of the Philippines Los Baños College, Laguna.
Journal of Virological Methods
|October 3, 1999
Abstract:
Polymerase chain reaction (PCR) primers were designed based on the sequence of a cloned fragment of the white spot virus (WSV) genome and were used to detect at least four geographic isolates of WSV from both experimentally- and naturally-infected shrimp. In addition to high specificity, the one-step and two-step PCR protocols were determined to have sensitivities of 10-100 pg and 100 femtograms respectively. The two-step PCR protocol is recommended as a very sensitive and specific alternative protocol to Western blot assay for the detection of WSV.