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Reconstruction of an active integron recombination site after integration of a gene cassette at a secondary site
H Segal1, M V Francia, J M Lobo
1Department of Medical Microbiology, University of Cape Town, South Africa.
Antimicrobial Agents and Chemotherapy
|October 3, 1999
Abstract:
As the site of insertion of the aadB gene cassette on pRAY, from a clinical isolate of Acinetobacter, is almost identical to the preferred site on integrons, the composite 59-base element (59-BE) associated with this cassette is potentially recombinationally active. By using a conduction assay to quantitate site activity, the 59-BE was recognized by integrase with high frequency, indicating that the composite site is recombinationally active.