Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel peptide-SH3 interaction.

A M Mongioví1, P R Romano, S Panni

  • 1Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan.

The EMBO Journal
|October 3, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Placental insufficiency markers to assess the risk of non-chromosomal genetic conditions in early-onset fetal growth restriction.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2026
Same author

Angiogenic factors and fetal Doppler for predicting adverse pregnancy outcome in early-onset small fetuses with and without pre-eclampsia.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2026
Same author

Infertility in an American Quarter Horse mare with cervical duplication.

Journal of equine veterinary science·2026
Same author

Cost-benefit analysis of first-trimester pre-eclampsia screening: comparison of multivariable Gaussian algorithm with and without placental growth factor.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2026
Same author

Re: Angiogenic factors alone or in combination with ultrasound Doppler criteria for risk classification among late-onset small fetuses with or without pre-eclampsia.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2025
Same author

Birth Cohort Study Identifies <i>Candida albicans</i> as a Risk Factor for Dental Caries.

Journal of dental research·2025

The Eps8 SH3 domain binds a novel PXXDY peptide motif, not the typical XPXXP sequence. This discovery reveals a new class of SH3 proteins with distinct signaling roles.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Protein Interactions

Background:

  • SH3 domains are crucial protein-protein interaction modules.
  • Canonical SH3 domains recognize the X-proline-X-X-proline (XPXXP) consensus motif.
  • Eps8 is a substrate of receptor and non-receptor tyrosine kinases.

Purpose of the Study:

  • To investigate the binding preference of the Eps8 SH3 domain.
  • To identify novel binding motifs for SH3 domains.
  • To characterize a new subfamily of SH3-containing proteins.

Main Methods:

  • Screening of phage-displayed random peptide libraries.
  • Mapping binding regions on Eps8 interactors.
  • Alanine scanning and in vitro cross-linking assays.

Related Experiment Videos

  • Expressed Sequence Tags database screening.

    • Main Results:

    • The Eps8 SH3 domain uniquely binds a proline-X-X-aspartate-tyrosine (PXXDY) consensus.
    • Three Eps8-related genes with distinct SH3 binding preferences were identified.
    • These genes form a phylogenetically distinct SH3 subfamily.

    Conclusions:

    • Eps8 SH3 domain defines a novel class of SH3 proteins.
    • This new subfamily does not bind canonical XPXXP peptides.
    • These proteins mediate distinct interactions within cellular signaling networks.