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Type II restriction endonucleases: structural, functional and evolutionary relationships
1Institute of Molecular Biology, Howard Hughes Medical Institute, Department of Physics, 1229 University of Oregon, Eugene, OR 97403-1229, USA.
Current Opinion in Chemical Biology
|October 6, 1999
Abstract:
Type II restriction endonucleases are a paradigm for site-specific cleavage of DNA. Recent structural analyses, in particular in the presence of various divalent metals, have shed new insight into the mechanisms of catalysis. In addition, during this past year the crystal structure determinations of MutH, lambda-exonuclease and FokI have revealed that these proteins are also members of the same family.