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Detection of salmonella in poultry using a silicon chip-based biosensor
K Dill1, L H Stanker, C R Young
1Molecular Devices Corporation, Sunnyvale, CA 94089, USA. KDill@CombiMatrix.com
Journal of Biochemical and Biophysical Methods
|October 8, 1999
Summary
A new Threshold Immunoassay System detects Salmonella typhimurium at low levels. This method shows high recovery rates in chicken carcass testing, offering a promising tool for food safety analysis.
Area of Science:
- Food Microbiology
- Immunotechnology
- Biosensors
Background:
- Accurate detection of Salmonella typhimurium is crucial for food safety.
- Traditional methods can be time-consuming and labor-intensive.
- Development of rapid and sensitive detection systems is needed.
Purpose of the Study:
- To evaluate the performance of a Threshold Immunoassay System for Salmonella typhimurium detection.
- To assess the system's sensitivity and applicability in food matrices.
Main Methods:
- Utilized a solution-based immunoassay with biotin and fluorescein labeled antibodies.
- Employed filtration-capture on a biotin-coated nitrocellulose membrane.
- Detection via a silicon chip-based light-addressable potentiometric sensor measuring pH changes from urease activity.
Main Results:
- Achieved detection of Salmonella typhimurium down to 119 Colony Forming Units (CFUs).
- Demonstrated a 90% recovery rate when testing fortified chicken carcass washings.
- Signal output reported as microV s(-1) based on monitored pH changes.
Conclusions:
- The Threshold Immunoassay System is a sensitive and effective method for detecting Salmonella typhimurium.
- The technique is suitable for analyzing Salmonella in chicken carcass washings.
- Care must be taken to avoid sample debris that can impede membrane flow and affect signal output.