[Predisposition to in vitro oxidation of LDL isolated from hypercholesterolemic patients. Interaction with arterial...

It is very well documented that cholesterol of the atherosclerotic plaques comes from LDL plasma particles and also a positive correlation between LDL plasma level and intensity of atherosclerotic lesions has been shown. Recent findings have revealed a direct relationship among arterial tissue atherogenic response and polyunsaturated fatty acids oxidation. The oxidized fatty acids within the low density lipoproteins makes these particles more atherogenic. In the present study the susceptibility of low density lipoprotein isolated from hypercholesterolemic patients to become oxidatively modified in vitro was investigated. The results show that the predisposition of hypercholesterolemic patients LDL to oxidation is fourfold increased, expressed in TBARS content (p < 0.0001) as compared to control LDL. Furthermore, the interactions of LDL with arterial proteoglycans apparently contribute to the accumulation of apo-B lipoproteins in atherogenesis. The hypercholesterolemic patients LDL shows higher values of proteoglycans-insolubilized LDL than LDL from controls.