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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1
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Influence of different cellular transcription factors on the regulation of varicella-zoster virus glycoproteins E

M Rahaus1, M H Wolff

  • 1Institute of Microbiology and Virology, University of Witten Herdecke, Germany.

Virus Research
|October 8, 1999
PubMed
Summary

This study reveals that common cellular transcription factors regulate Varicella-zoster virus (VZV) late gene expression, specifically for glycoproteins E and I. These findings offer insights into VZV replication and pathogenesis.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Varicella-zoster virus (VZV) glycoproteins E (gE) and I (gI) are crucial components of the viral envelope and host cell surface.
  • Expression of gE and gI is known to be activated by VZV immediate-early proteins IE4 and IE62.

Purpose of the Study:

  • To investigate the regulatory mechanisms controlling VZV gE and gI gene expression.
  • To identify cis-acting elements and trans-acting factors involved in VZV late gene regulation.

Main Methods:

  • Analysis of intergenic regions (putative promoters) between VZV ORFs 66/67 (gI) and 67/68 (gE).
  • Mapping of the transcriptional start site for gE.
  • Construction and testing of reporter gene constructs utilizing VZV promoter regions.
  • In cis-trans interaction studies with transcription factors (LAP/LIP, Sp1, YY1, NF-E2).
  • Protein-DNA binding assays using VZV intergenic regions and cellular extracts.

Main Results:

  • Identification of eukaryotic cis-elements (TATA, CAAT motifs) in VZV gE and gI promoter regions.
  • Demonstration of measurable influence of overexpressed transcription factors on reporter gene expression.
  • Evidence of binding by 32 and 18 kDa proteins to gE and gI intergenic regions.

Conclusions:

  • Common cellular transcription factors play a role in regulating VZV late gene expression.
  • These findings contribute to understanding VZV gene regulation and viral pathogenesis.