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Related Experiment Videos

Cyclic strain induces redox changes in endothelial cells.

J J Cheng1, B S Wung, Y J Chao

  • 1Cardiovascular Division, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.

The Chinese Journal of Physiology
|October 8, 1999
PubMed
Summary
This summary is machine-generated.

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Cyclic strain on endothelial cells increases reactive oxygen species (ROS). This triggers antioxidant responses and heme oxygenase-1 (HO-1) gene induction, crucial for cellular adaptation.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Oxidative Stress Research

Background:

  • Endothelial cells (ECs) are vital for vascular health.
  • Cyclic strain is a physiological stimulus impacting ECs.
  • Reactive oxygen species (ROS) act as signaling molecules in ECs.

Purpose of the Study:

  • To investigate the impact of cyclic strain on EC antioxidant activity.
  • To examine the role of ROS in HO-1 gene expression in ECs.
  • To elucidate the mechanism linking ROS, glutathione levels, and HO-1 induction.

Main Methods:

  • Subjecting ECs to cyclic strain.
  • Measuring lipid peroxidation and low-density lipoprotein oxidation.
  • Assessing superoxide dismutase and glutathione peroxidase activities.

Related Experiment Videos

  • Quantifying glutathione (GSH) and oxidized glutathione (GSSG) levels.
  • Analyzing heme oxygenase-1 (HO-1) expression.
  • Utilizing catalase and free radical scavengers for inhibition studies.

    • Main Results:

    • Cyclic strain increased ROS, lipid peroxidation, and LDL oxidation in ECs.
    • Superoxide dismutase and glutathione peroxidase activities were upregulated post-strain.
    • A transient decrease in GSH and increase in GSSG levels were observed.
    • Strain-induced HO-1 expression was dependent on ROS and GSH levels.
    • Catalase and N-acetyl-cysteine treatments modulated HO-1 induction.

    Conclusions:

    • Cyclic strain-induced ROS transiently increase glutathione peroxidase activity, decreasing GSH levels in ECs.
    • The reduction in GSH is a critical factor for HO-1 gene induction.
    • These findings highlight a novel ROS-mediated signaling pathway in ECs under mechanical stress.