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Updated: Aug 9, 2026
Comparative Lesions Analysis Through a Targeted Sequencing Approach
Published on: November 5, 2019
[Mutation analysis of p16 gene in non-small cell lung carcinomas]
1Department of Medical Genetics, Harbin Medical University, Harbin, Henongjiang, 150086 P. R. China. fusb@ems.hrbmu.edu.cn
Objective:
To investigate the role of p16 gene in the tumorigenesis of non-small cell lung carcinomas (NSCLC).
Methods:
Homozygous deletion and mutation of exon 2 of p16 genes in 40 NSCLC tissues were analyzed using PCR and dsDNA direct sequencing technique.
Results:
In 2 NSCLC tissues, homozygous deletions of p16 gene were detected; in 14 NSCLC tissues, 19 point mutations and a frameshift were detected.
Conclusion:
Point mutations of p16 gene were frequent and might play a role in the initiation and progression of NSCLC. Nt380 in this gene was a hot spot of mutation.
